关于异基因生物疗的疗效（论文内容已经放到206楼）

kikitty

<p>第一次在奇迹网发帖，呵呵。</p><p>之前看到关于崔征博士的报道，不管可行性大小，真的是很激动。然后，就看到球的这个帖子，接着在学校数据库里找到这篇论文，只有CAJ和PDF两种格式，本来想转成文档格式上传，但文中表格之类转成文档会错位~所以只能把摘要贴上来~如果有需要的话，我也可以帮忙发到邮箱~<span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">&nbsp;</span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">摘要</span><span lang="EN-US"><span style="mso-spacerun: yes;"><font face="Times New Roman">&nbsp; </font></span></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">目的</span><span lang="EN-US"><font face="Times New Roman">: </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">初步探讨</span><span lang="EN-US"><font face="Times New Roman">IL- 2 </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">短期活化的</span><span lang="EN-US"><font face="Times New Roman">HLA </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">半相合的异体外周血单个核细胞</span><span lang="EN-US"><font face="Times New Roman">( allo- mPBMCs) </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">治疗晚期转移性</span><span lang="EN-US"><font face="Times New Roman">/</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">化疗抗性实体瘤的可行性。方法</span><span lang="EN-US"><font face="Times New Roman">: 11 </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">例晚期转移性</span><span lang="EN-US"><font face="Times New Roman">/</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">化疗抗性恶性实体瘤患者</span><span lang="EN-US"><font face="Times New Roman">, </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">供者为与患者</span><span lang="EN-US"><font face="Times New Roman">HLA </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">半相合直系亲属。供者经</span><span lang="EN-US"><font face="Times New Roman">10</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">μ</span><span lang="EN-US"><font face="Times New Roman">g/kg</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">·</span><span lang="EN-US"><font face="Times New Roman">d G- CSF </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">动员</span><span lang="EN-US"><font face="Times New Roman">3 </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">天后采集</span><span lang="EN-US"><font face="Times New Roman">BMC, </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">体外用大剂量</span><span lang="EN-US"><font face="Times New Roman">rhIL- 2 </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">培养后回输患者。观察治疗后临床反应</span><span lang="EN-US"><font face="Times New Roman">,</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">采用流式细胞仪、</span><span lang="EN-US"><font face="Times New Roman">LDH </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">和</span><span lang="EN-US"><font face="Times New Roman">ELISA </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">试剂盒检测动员、活化前后表型</span><span lang="EN-US"><font face="Times New Roman">, IL- 2 </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">短期活化前后的非特异性杀瘤活性以及患者回输前后血清中各种细胞因子的含量变化。结果</span><span lang="EN-US"><font face="Times New Roman">: 11 </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">例患者回输的</span><span lang="EN-US"><font face="Times New Roman">HLA </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">半相合</span><span lang="EN-US"><font face="Times New Roman">allo- mPBMCs </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">总数达</span><span lang="EN-US"><font face="Times New Roman">( 2.5</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">～</span><span lang="EN-US"><font face="Times New Roman">4.3) </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">×</span><span lang="EN-US"><font face="Times New Roman">1010</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">。体外经</span><span lang="EN-US"><font face="Times New Roman">G- CSF </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">动员后</span><span lang="EN-US"><font face="Times New Roman">T </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">细胞</span><span lang="EN-US"><font face="Times New Roman">, </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">尤其是</span><span lang="EN-US"><font face="Times New Roman">CD4+T </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">细胞显著下降</span><span lang="EN-US"><font face="Times New Roman">, </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">而</span><span lang="EN-US"><font face="Times New Roman">NK </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">细胞比例显著上升</span><span lang="EN-US"><font face="Times New Roman">; </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">经大剂量</span><span lang="EN-US"><font face="Times New Roman">IL- 2 </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">短期活化后</span><span lang="EN-US"><font face="Times New Roman">, </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">各种细胞亚群比例未见改变</span><span lang="EN-US"><font face="Times New Roman">, </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">但活化细胞</span><span lang="EN-US"><font face="Times New Roman">( </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">包括</span><span lang="EN-US"><font face="Times New Roman">CD69+</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">和</span><span lang="EN-US"><font face="Times New Roman">CD25+</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">细胞</span><span lang="EN-US"><font face="Times New Roman">) </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">亚群比例显著升高。体外实验中可观察到</span><span lang="EN-US"><font face="Times New Roman">IL- 2 </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">短期活化的</span><span lang="EN-US"><font face="Times New Roman">allo- mPBMCs </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">具有类似</span><span lang="EN-US"><font face="Times New Roman">LAK </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">样的较高的非特异性杀瘤活性。经过治疗</span><span lang="EN-US"><font face="Times New Roman">, 11 </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">例患者血清中</span><span lang="EN-US"><font face="Times New Roman">Th1 </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">类细胞因子</span><span lang="EN-US"><font face="Times New Roman">, </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">如</span><span lang="EN-US"><font face="Times New Roman">IL- 2</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">、</span><span lang="EN-US"><font face="Times New Roman">IL- 12</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">、</span><span lang="EN-US"><font face="Times New Roman">IFN- </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">γ、</span><span lang="EN-US"><font face="Times New Roman">TNF- </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">α含量明显升高</span><span lang="EN-US"><font face="Times New Roman">, </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">而</span><span lang="EN-US"><font face="Times New Roman">Th2 </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">类细胞因子</span><span lang="EN-US"><font face="Times New Roman">, </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">如</span><span lang="EN-US"><font face="Times New Roman">TGF- </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">β含量明显下降。治疗后</span><span lang="EN-US"><font face="Times New Roman">8 </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">例观察到临床症状的缓解</span><span lang="EN-US"><font face="Times New Roman">, </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">或肿瘤标志物的降低</span><span lang="EN-US"><font face="Times New Roman">, </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">或影像学资料的改善</span><span lang="EN-US"><font face="Times New Roman">, </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">其中</span><span lang="EN-US"><font face="Times New Roman">1 </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">例治疗后</span><span lang="EN-US"><font face="Times New Roman">4 </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">个月达到部分缓解</span><span lang="EN-US"><font face="Times New Roman">, </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">而</span><span lang="EN-US"><font face="Times New Roman">3 </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">例出现进展</span><span lang="EN-US"><font face="Times New Roman">, </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">其中</span><span lang="EN-US"><font face="Times New Roman">2 </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">例死亡。结论</span><span lang="EN-US"><font face="Times New Roman">: IL- 2 </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">短期活化的</span><span lang="EN-US"><font face="Times New Roman">HLA </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">半相合的</span><span lang="EN-US"><font face="Times New Roman">allo- mPBMCs </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">具有明显的抗肿瘤效应</span><span lang="EN-US"><font face="Times New Roman">, </font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">为治疗晚期转移性</span><span lang="EN-US"><font face="Times New Roman">/</font></span><span style="FONT-FAMILY: 宋体; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman';">化疗抗性实体瘤提供了新的希望。</span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt;"><span lang="EN-US"><p><font face="Times New Roman">&nbsp;</font></p></span></p>

kikitty

<p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-pagination: widow-orphan;"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-family: 宋体; mso-font-kerning: 0pt;">以上是文章摘要。<span lang="EN-US"><p></p></span></span></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-family: 宋体; mso-font-kerning: 0pt;"><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-pagination: widow-orphan;"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-family: 宋体; mso-font-kerning: 0pt;">文中提到选取的是<span lang="EN-US">2002 </span>年<span lang="EN-US">11</span>月～<span lang="EN-US">2003 </span>年<span lang="EN-US">12</span>月间进行活化<span lang="EN-US">HLA </span>半相合的<span lang="EN-US">allo- mPBMCs </span>治疗的肿瘤患者<span lang="EN-US">11</span>例<span lang="EN-US">, </span>所有患者临床诊断符合<span lang="EN-US">IV </span>期恶性实体瘤<span lang="EN-US">, </span>均不宜化疗或化疗无效。这个时间段距离现在也有３年半的时间了，不知道现在的临床效果如果？我想请问球，如果化疗有效的话，这项疗法是否也能激发患者本身的免疫能力？此外，你说的<span lang="EN-US">2.9</span>万费用，是指未报医保的所有费用吗？～～谢谢 <span lang="EN-US"><p></p></span></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-pagination: widow-orphan;"><span lang="EN-US" style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-family: 宋体; mso-font-kerning: 0pt;">&nbsp; <p></p></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-pagination: widow-orphan;"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-bidi-font-family: 宋体; mso-font-kerning: 0pt;">说实话，我现在几乎迫不及待想让我亲爱的妈妈去做这项疗法。我妈妈是右肺上尖腺癌，已经是局部晚期，扩散至胸膜，锁骨上也有少数淋巴结。现在化疗三个疗程结束，效果还是很不错的，医生说已经达到<span lang="EN-US">R</span>等级了。但<span lang="EN-US">R</span>远远不够，我希望可以临床治愈，甚至是完全治愈。期望这项技术可以尽快成熟，好给我们更多的希望。<span lang="EN-US"><p></p></span></span></p><p class="MsoNormal" align="left" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-pagination: widow-orphan;"></p></span>

[此贴子已经被作者于2007-9-23 23:49:19编辑过]

球

<p>kikitty，你好，非常赞赏你对母亲的孝够心。也很赞赏你对你妈妈能够完全治愈的信心。</p><p><span lang="EN-US">HLA </span>半相合的治疗方式是一种先进的生物治疗方式，他和化疗是不冲突的，无论化疗效果如何都可以使用这种治疗方式。</p><p>关于2.9万的费用是指的报销前的费用。</p><p>不过你也不能对这个治疗抱有太大的期望。</p><p>论文你也看到了，相信你会对这项治疗有一个客观的评价。</p>

hj_2002nx

请给我也发一套,谢谢!

hj_2002nx

请给我发一套，邮箱: <a href="mailto:hj_2002nx@163.com">hj_2002nx@163.com</a>谢谢！

kikitty

<p>球，你好～～谢谢你的回答～～</p><p>说实话，我真的真的是很不孝。妈妈查出是肺癌之后，一直瞒了我一个月，怕影响我的学习。她是7月13号的胸透查出有阴影，16号确诊，而那段时间我一直在学校忙导师的课题，等到8月10号才知道这个消息。当时真的恨死自己了。那一个月里，我几乎是不断的短信和电话给我妈妈，为什么就一点感觉不出来她的异样和强颜欢笑呢？有时真恨不得抽死自己。</p><p>好在妈妈的化疗效果很不错，两疗程结束的时候，CT显示肿瘤缩小了三份之一。那天听到主治医生说，已经PR了。现在第三个疗程结束，接着停疗21天，再开始第四疗程。</p><p>我有时候觉得妈妈能够一直这样好下去，因为她的身体状况、精神状态什么都很好；但有的时候又觉得很茫然，因为看了太多离去的人们。昨晚一直在看芬芳姐姐的BLOG，那么年轻坚强乐观的姐姐就这样离开了，生活真的非常残忍。我不知道我什么时候也要面临这一天，现在看着妈妈，忽然就很想抱抱她亲亲她，我真的不知道以后还有没有这样的机会了。对每个孩子来说，世界上除了妈妈之外，还有谁可以不计回报的深爱着自己？为什么要在我还没有能力报答她之前，就从我身旁带走她？！如果上天听得见我们的声音，就请给我们最爱的亲人多一点时间，再多一点，然后一直延长下去。现在只希望妈妈的病情可以完全控制住，然后一天一天好起来，新的医疗技术可以尽快的出现，也希望大家最爱的亲人都能够早日康复！</p>

kikitty

<p><strong><font face="Verdana" color="#61b713">TO：hj_2002nx</font></strong></p><p><strong><font face="Verdana" color="#61b713">已经发到你的邮箱了~~</font></strong></p>

qwee

<p><font face="Verdana" color="#61b713"><strong>kikitty兄弟或球兄弟能给我发一份吗？<a href="mailto:s9101527@163.com">s9101527@163.com</a></strong></font></p><p><font face="Verdana" color="#61b713"><strong>这个对小细胞肺癌效果怎样？能有办法让病人不去吗</strong>&nbsp;？谢谢了</font></p>