《病急乱投医》--三联生活周刊

jimmy112199

这是检索到的文摘，是2008年4月发表的。文中对人体子宫癌进行研究。DCA对低，中恶度癌有效，但对高恶度癌无效。

Dichloroacetate induces apoptosis in endometrial cancer cellsstar, open

Jason Y.Y. Wonga, Gordon S. Hugginsb, Marcella Debiddad, Nikhil C. Munshid and Immaculata De Vivoa, c, Corresponding Author Contact Information, E-mail The Corresponding Author
aChanning Laboratory, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston Massachusetts, USA bMolecular Cardiology Research Institute, Tufts-New England Medical Center, Boston Massachusetts, USA cProgram in Molecular and Genetic Epidemiology, Harvard School of Public Health, Boston Massachusetts, USA dThe Jerome Lipper Multiple Myeloma Center, Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston Massachusetts, USA

Received 21 November 2007.
Available online 18 April 2008.

This article is not included in your organization's subscription. However, you may be able to access this article under your organization's agreement with Elsevier.
Abstract
Purpose

A recent landmark study demonstrated that Dichloroacetate (DCA) treatment promoted apoptosis in lung, breast, and glioblastoma cancer cell lines by shifting metabolism from aerobic glycolysis to glucose oxidation coupled with NFAT-Kv1.5 axis remodeling. The objective of this study was to determine whether DCA induces apoptosis in endometrial cancer cells and to assess apoptotic mechanism.
Methods

A panel of endometrial cancer cell lines with varying degrees of differentiation was treated with DCA and analyzed for apoptosis via flow cytometry. Biological correlates such as gene expression, intracellular Ca2+, and mitochondrial membrane potential were examined to assess apoptotic mechanism.
Results

Initiation of apoptosis was observed in five low to moderately invasive cancer cell lines including Ishikawa, RL95-2, KLE, AN3CA, and SKUT1B while treatment had no effect on non-cancerous 293T cells. Two highly invasive endometrial adenocarcinoma cell lines, HEC1A and HEC1B, were found to be resistant to DCA-induced apoptosis. Apoptotic responding cell lines had a significant increase in early and late apoptotis, a decrease in mitochondrial membrane potential, and decreased Survivin transcript abundance, which are consistent with a mitochondrial-regulated mechanism. DCA treatment decreased intracellular calcium levels in most apoptotic responding cell lines which suggests a contribution from the NFAT-Kv1.5-mediated pathway. DCA treatment increased p53 upregulated modulator of apoptosis (PUMA) transcripts in cell lines with an apoptotic response, suggesting involvement of a p53-PUMA-mediated mechanism.
Conclusions

Dichloroacetate effectively sensitizes most endometrial cancer cell lines to apoptosis via mitochondrial, NFAT-Kv1.5, and PUMA-mediated mechanisms. Further investigation of the cancer therapeutic potential of DCA is warranted.

不平则鸣

到底能用还是不能用，为何没有一个权威的说法

mjl

现在用dca治疗本就属自疗，就像蟾蜍，有权威的说法吗？能用还是不能用，我们可以选择，但是否有效，只有试过才知道啊。

jimmy112199

这是加拿大阿尔伯塔大学10月2日的新结果，对脑癌病人试药的结果很有希望。但教授不建议没有医生指导下自己服用

Letter from Dr. Evangelos Michelakis
August 02, 2008



Dear Friends,



This is a short update on our DCA project at the University of Alberta.



We would like to express our gratitude and appreciation for your support in our fund raising efforts. Through donations on this web page and philanthropic foundations we have been able to expand our basic science efforts as well as initiated and run two clinical trials in Edmonton. In collaboration with the Cross Cancer Institute and the Alberta Cancer Board, we are running clinical trials in patients with solid tumours that have failed standard therapies as well as in patients with malignant brain tumours. The objectives of these trials is to determine the safety of DCA as a novel therapy for cancer. We are trying to determine the optimal dose and to monitor potential adverse effects, such as drug interactions and toxicities. This is necessary to do before embarking on more definitive trials to test the effectiveness of this drug in the treatment of cancer. We have enrolled more than half of our target numbers of patients in these trials and we are gaining invaluable experience. Both the progress and the preliminary results are promising, although the detailed outcomes of these trials will be published in medical journals, after their completion.



We are very encouraged by these early results, and are now preparing to initiate additional clinical trials, in Edmonton and in collaboration with other Universities in around the world.



We are gratified to see that other researchers have begun to share our excitement with DCA. Several recent studies have reported findings similar to ours. These include some work suggesting that DCA has anti-cancer effects in endometrial and prostate cancer (see the references at the end of this letter). However a word of caution, these experiments were not done in patients, but rather in animals and test tubes, and so the applicability of these results to human disease has not been determined. They make however, the need for clinical trials even more pressing. We also reiterate that in the absence of knowledge about the safety and effectiveness of this drug in people it is unwise to be taking this agent unsupervised and outside of a clinical trial.



On behalf of the many people here at the University of Alberta that continue to work on DCA,



Thank you very much!



Recent medical reports on DCA:



Journal: Gynecol Oncol. 2008 Jun;109(3):394-402
Title: Dichloroacetate induces apoptosis in endometrial cancer cells.
Authors: Wong JY, Huggins GS, Debidda M, Munshi NC, De Vivo I. Channing Laboratory, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA



Journal : Prostate. 2008 Aug 1;68(11):1223-3
Title: Dichloroacetate (DCA) sensitizes both wild-type and over expressing Bcl-2 prostate cancer cells in vitro to radiation.
Authors: Cao W, Yacoub S, Shiverick KT, Namiki K, Sakai Y, Porvasnik S, Urbanek C, Rosser CJ. Department of Urology, University of Florida, Gainesville, Florida, USA

Journal: Proceedings of the National Academy of Sciences, PNAS 2007 104:9445-9450
Title: Metabolic targeting of hypoxia and HIF1 in solid tumors can enhance cytotoxic chemotherapy
Authors: Rob A. Cairns, Ioanna Papandreou, Patrick D. Sutphin, and Nicholas C. Denko Stanford University, Palo Alto, CA, USA

爱的力量

小白这篇资料非常的中立，客观。也给我对于DCA的看法有所改观，药厂追逐利益方面是让人无可奈何的寒心，如果可以我们都愿意捐钱给她们研究，至于拖到现在DCA还没有走入正式临床，我们唯一的选择就是自救，碰运气了。谢谢MJL的好消息！祝福你的家人！

留住生命

DCA的常用名是什么？

mjl

就是二氯乙酸钠，但是有化学级的和药品级的，
纯度高副作用才会小些。

碱性健康人

看了mjl妈妈的经历，顶一下！
希望有更多服用过DCA的病友讲一下服用的效果和经验，给大家更多的信心！
另外，有谁能讲一下购买DCA的途径吗？希望能知道多一点这方面的信息。